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pdgfaa  (R&D Systems)


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    R&D Systems pdgfaa
    Pdgfaa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 158 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pdgfaa/Recombinant+Human+PDGF-AA+Protein%2C+CF/pm41486410-345-2-3
    Average 95 stars, based on 158 article reviews
    pdgfaa - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    other:

    Article Title: Microglial HVCN1 Deficiency Improves Movement and Survival of SOD1 G93A ALS Mice by Enhancing Microglial Migration and Neuroprotection
    Article Snippet: 10 ng/ml PDGFaa (R & D system, 221‐AA‐010) was added for OPC culture, whereas 40 ng/ml T3 (Sigma, T‐074) was added to induce differentiation into mature oligodendrocytes.

    Article Title: Microglial HVCN1 Deficiency Improves Movement and Survival of SOD1 G93A ALS Mice by Enhancing Microglial Migration and Neuroprotection.
    Article Snippet: 10 ng/ml PDGFaa (R & D system, 221-AA-010) was added for OPC culture, whereas 40 ng/ml T3 (Sigma, T-074) was added to induce differentiation into mature oligodendrocytes.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Comparative release of growth factors from PRP, PRF, and advanced-PRF.
    Article Snippet: Objectives The use of platelet concentrates has gained increasing awareness in recent years for regenerative procedures in modern dentistry.. The aim of the present study was to compare growth factor release over time from platelet-rich plasma (PRP), platelet-rich fibrin (PRF), and a modernized protocol for PRF, advanced-PRF (A-PRF).. Materials and methods Eighteen blood samples were collected from six donors (3 samples each for PRP, PRF, and APRF).



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    (a) Inpp5e +/‒ and Inpp5e ‒/‒ MEFs were stimulated <t>with</t> <t>PDGF-AA</t> or FBS for the indicated times and cell lysates analyzed by Western blot with the indicated antibodies. Actin was used as loading control. (b-c) Quantitations of normalized pAKT/AKT ratios from n=4 (S473) or n=3 (T308) independent experiments, including the one in (a). (d) Inpp5e +/+ , Inpp5e +/‒ and Inpp5e ‒/‒ MEFs cultured with or without FBS were analyzed by Western blot with the indicated antibodies. Tubulin was used as loading control and phospho-retinoblastoma (pRb) as cell cycle marker. Note INPP5E-dependence of PDGFRα receptor levels in serum-starved MEFs.
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    (a) Inpp5e +/‒ and Inpp5e ‒/‒ MEFs were stimulated <t>with</t> <t>PDGF-AA</t> or FBS for the indicated times and cell lysates analyzed by Western blot with the indicated antibodies. Actin was used as loading control. (b-c) Quantitations of normalized pAKT/AKT ratios from n=4 (S473) or n=3 (T308) independent experiments, including the one in (a). (d) Inpp5e +/+ , Inpp5e +/‒ and Inpp5e ‒/‒ MEFs cultured with or without FBS were analyzed by Western blot with the indicated antibodies. Tubulin was used as loading control and phospho-retinoblastoma (pRb) as cell cycle marker. Note INPP5E-dependence of PDGFRα receptor levels in serum-starved MEFs.
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    (a) Inpp5e +/‒ and Inpp5e ‒/‒ MEFs were stimulated with PDGF-AA or FBS for the indicated times and cell lysates analyzed by Western blot with the indicated antibodies. Actin was used as loading control. (b-c) Quantitations of normalized pAKT/AKT ratios from n=4 (S473) or n=3 (T308) independent experiments, including the one in (a). (d) Inpp5e +/+ , Inpp5e +/‒ and Inpp5e ‒/‒ MEFs cultured with or without FBS were analyzed by Western blot with the indicated antibodies. Tubulin was used as loading control and phospho-retinoblastoma (pRb) as cell cycle marker. Note INPP5E-dependence of PDGFRα receptor levels in serum-starved MEFs.

    Journal: bioRxiv

    Article Title: INPP5E interactome reveals novel connections to growth factor signaling

    doi: 10.64898/2026.02.13.705725

    Figure Lengend Snippet: (a) Inpp5e +/‒ and Inpp5e ‒/‒ MEFs were stimulated with PDGF-AA or FBS for the indicated times and cell lysates analyzed by Western blot with the indicated antibodies. Actin was used as loading control. (b-c) Quantitations of normalized pAKT/AKT ratios from n=4 (S473) or n=3 (T308) independent experiments, including the one in (a). (d) Inpp5e +/+ , Inpp5e +/‒ and Inpp5e ‒/‒ MEFs cultured with or without FBS were analyzed by Western blot with the indicated antibodies. Tubulin was used as loading control and phospho-retinoblastoma (pRb) as cell cycle marker. Note INPP5E-dependence of PDGFRα receptor levels in serum-starved MEFs.

    Article Snippet: Other reagents included GFP-Trap_MA beads (Proteintech, gtma), mouse anti-Flag M2 magnetic beads (Sigma, M8823), sodium orthovanadate (Alfa Aesar, J60191) and the following recombinant human growth factors: PDGF-AA (Proteintech, HZ-1215), TGF-β1 (R&D Systems, 240-B), Insulin (Gibco, 12585014), IGF-I (Proteintech, HZ-1322) and EGF (Proteintech, HZ-1326).

    Techniques: Western Blot, Control, Cell Culture, Marker